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EV71 testing for HFMD

24 April 2015

Dear Doctors,

Hand, foot and mouth disease (HFMD) and herpangina are caused by different enteroviruses. Although the diagnosis is usually made clinically without identifying the virus, it is important to do so because enterovirus 71 (EV71) can potentially cause severe neurological disease. Malaysia experiences cyclical patterns of EV71 outbreaks every 3 years, and as the last outbreak occurred in 2012, an outbreak may occur in 2015.

We are currently validating an EV71 diagnosis IgM kit, which will allow diagnosis of EV71 as a cause of HFMD. We would like to extend the service to more patients with HFMD or herpangina to ensure the diagnosis kit is good before its use in routine diagnosis. Your participation in this research project is greatly appreciated. The benefits to your patients are that they will have 2 tests to identify the cause of the HFMD, including the gold standard of culture/PCR on the swab, at NO COST.

If you need more information, please contact us at:

Dr.Tan Chee Wah
Email: tcwah@um.edu.my
Tel: 03-79675752 or 016-9045672

Dr. Chan Yoke Fun
Email: chanyf@um.edu.my
Tel: 03-79676677 or 017-2092676

Specimen Collection (Please collect blood and throat/mouth ulcer swab)

1. Provide clinic and patient information (Fill in the form).

2. Only plasma specimen (collected in heparin tube) can be used with this assay. Ideal volume: 2-3 ml

3. Throat or mouth ulcer swab- Place the swab in viral transport medium. If not available, use gel-based transport swab.

Transportation and Storage

1. Store specimens at 2-8°C.

2. Address the specimens to Dr Chan Yoke Fun and send the specimens to Lab A2-24 (Lab 6), Department of Medical Microbiology, Faculty of Medicine, University Malaya, 50603 Kuala Lumpur.

Expected Results

1. The EV71 IgM test result will be released in two working days.

2. Expected results: EV71 IgM positive or EV71 IgM negative.

3. The swab culture will identify most enteroviruses but will take several weeks.

4. Cost of the tests: FREE

Please download the letter below...


  arrowHFMD Letter to Drs.pdf (English - pdf - 229 Kb)   



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